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Exerc Sci > Volume 12(4); 2003 > Article

Exercise Science 2003;12(4): 679-692.

탈지적 운동과 항산화제섭취가 산화적 손상과 항산화효소의 활성 및 항산화효소의 유전자 발현에 미치는 영향

이왕록

Iowa State University

ABSTRACT

Lee W.L. Effects of Exhaustive Exercise and antioxidants supplementation on Oxidative damage, Antioxidants Enzyme Activity and mRNA expression in Rats. Exercise Science, 12(4): 679-692, 2003. The purpose of this present study was to investigate the effect of a long-term antioxidants supplementation on oxidative damage of heart and antioxidant enzyme activity of skeletal muscle immediately after exhaustive exercise in rats. Forty male Sprague-Dawley rats(4 weeks old) were used as subjects. The subjects were divided into 4 groups such as, CON(control group), NAE(non antioxidants exercise group), ACantioxidants control group) or AE(antioxidants exercise group). Antioxidants group(AC & AE) fed the general food and the mixed antioxidants which were consist of vitamin E(200IU/kg/day), vitamin C(50mg/rat/day), vitamin B6(250ug/kg/day), β-carotene(300mg/kg/day) and selenomethionine (0.1mg/mg/day). After the diet experiment for 16 weeks, exercise groups(NAE & AE) completed acute treadmill running(speed increased gradually to 25m/min, 15% uphill grade, ～50±10min) until exhaustion. Immediately after exhaustive treadmill exercise, all the rats were sacrificed then, MDA of heart, SOD of soleus, GPx of gastrocnemius and GR of gastrocnemius were assayed. For the gene expression of antioxidant enzyme, Cu/Zn-SOD mRNA and Mn-SOD mRNA were assayed by RT-PCR in heart, lung, liver and soleus. As a rusult on antioxidant enzyme activity, exhaustive exercise increased MDA product by 40%, but a long-term antioxidants supplementation decreased significantly MDA product by 30% in the heart. Further, antioxidants supplementation increased significantly the GPx and GR activity of gastrocnemius muscle after exhaustive exercise(p<0.05). On the result of antioxidant enzyme gene expression, it seemed that Cu/Zn-SOD mRNA was increased by bothe exhaustive exercise and antioxidants supplementation.

Key words: Antioxidants, Exhaustive exercise, Antioxidants enzyme, mRNA* 이 논문은 2001년 학술진흥재단의 지원에 의하여 연구되었음. (KRF-2001-037-IB0002)