| 운동 강도의 차이가 고지방 식이 쥐의 골격근 내 지방 독성과 염증성 사이토카인 신호체계에 미치는 영향 |
| 정현령, 강호율 |
| 경북대학교 |
| Effects of different training intensities on lipotoxicity and pro-inflammatory cytokine signalling pathway of skeletal muscle in high-fat |
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| ABSTRACT |
PURPOSE:
The purpose of this study was to determine the effects of different training intensities on lipotoxicity and proinflammatory cytokine signal pathway of skeletal muscle in high-fat fed rats.
METHODS:
Forty rats were randomly divided into five groups: Normal control diet group (NC), high fat diet group (HF), high fat diet+low-intensity exercise group (HFLE, 22 m/min, 60 min, 6 days/week), high fat diet+moderate-intensity exercise group (HFME, 26 m/min, 51 min), and high fat diet+high-intensity exercise group (HFHE, 30 m/min, 46 min). After 4 weeks of high fat diet and endurance exercise training, the free fatty acid (FFA) concentrations were determined in plasma. Intramuscular triglyceride (TG), TNF-α, IKKα, IKKβ, p-IKKα/βser176/180, IκBα contents were measured in soleus muscle.
RESULTS:
After 4weeks treatments, Plasma FFA level and muscle TG contents were significantly lower in the three exercise (EX) groups than those in HF (P<0.05) The protein expressions of TNFα, IKKα, IKKβ and p-IKKα/βser176/180 were significantly lower in the HFLE, HFME, and HFHE groups compared to the HF group (P<0.05). Expression of IκBα in the three EX groups were significantly higher compared to that in HF (P<0.05).
CONCLUSIONS:
The results of this study suggested that the elevation of inflammatory cytokine protein expression due to the high-fat feeding significantly deteriorated the lipotoxicity and insulin resistance of skeletal muscle in rats, but this deterioration was significantly attenuated by aerobic training. However, the effect of different training intensities was not different in the expression of inflammatory cytokine protein in the muscle. |
| Key words:
high fat diet, lipotoxicity, TNF-α, IKK, IκBα |
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