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Exerc Sci > Volume 14(3); 2005 > Article
Exercise Science 2005;14(3): 359-370.
대퇴부위 저항성 트레이닝 후 프로테오믹스(proteomics) 방법에 의한 혈청과 근육조직 내 단백질 발현 분석
The effect of thigh muscle resistance training on serum and muscle protein profiles by proteomic analysis in thigh muscle
Kim, H.J. The effect of thigh muscle resistance training on serum and muscle protein profiles by proteomic analysis in thigh muscle. Exercise science. 14(3): 359-370, 2005. The present study was performed to investigate the effect of 12 weeks of resistance training on skeletal muscle and serum protein profiles of thigh muscle in five healthy volunteers (26-30yrs). Percutaneous muscle biopsies and computed tomography were obtained from the same portion of thigh muscle at pre- and posttraining. Blood sampling after overnight fast was taken at pre and post of training. Resistance training regimen was composed of three kinds of weight lifting exercise (squart, leg press, leg extension; 2 sessions/wk, 4 sets/exercise, 8RM/set, 2 times/wk) for 12weeks. Protein profiles were compared using by two-dimensional electrophoresis (2DE) technique and the differentially expressed spots were identified by matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) analysis. Computed tomographic scans revealed the increase of the thigh muscle cross sectional area (CSA, 192.80± 6.50cm2 vs 215.15±5.55cm2, p<.05). The significantly increased (more than 200%) protein profiles in serum were hemopexin precursor, human albumin, haptoglobin, immunoglobulin gamma-1 heavy chain constant region, and complement C4A precursor. On the other hand up regulated spots were NADH dehydrogenase Fe-S protein 3, heat shock 27kDa protein 1, actin alpha 1 skeletal muscle protein, and ubiquitin in muscle tissue.These results suggest that resistance exercise training, which increase muscle CSA by 11.6%, is associated with quantitave changes of protein profiles and these effects are independent from changing pattern of serum protein profiles.
Key words: resistance training, proteomic analysis, 2DE (2- dementional electro phoresis), MALDI-TOF (matrix-assisted laser desorption/ionization-time-of flight)
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