| 일회성 지구성운동 시 MEF2를 통한 PPARδ의 GLUT4 생합성 조절 |
| 김상현1, 김기진1, 정수련2, 고진호1 |
1계명대학교
2경주대학교 |
| Control of GLUT4 biogenesis through PPARδ and MEF2 axis with single bout of endurance exercise in rodent skeletal muscle |
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| ABSTRACT |
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There is increasing evidence that the regulator of skeletal muscle metabolism, PPARδ is associated with GLUT4 biogenesis. But, whether acute endurance exercise affects PPARδ and the mechanism of GLUT4 biogenesis has not been clearly identified. The expression of factors related to GLUT4 biogenesis must be of the same pattern as GLUT4 expression with endurance exercise. GLUT4 expression is increased by acute endurance exercise. But, PPARδ expression by acute endurance exercise is unclear. PPARδ was shown to induce the expression of PGC-1α in skeletal muscle. Since both are increased with acute endurance exercise, it is difficult to determine whether one or both, induce GLUT4 biogenesis in skeletal muscle. This study measured PPARδ expression in skeletal muscle by acute endurance exercise and confirmed the effect of PPARδ on GLUT4 biogenesis in PGC-1α KO mice. Sixteen rats were randomly assigned into 2 groups(Sedentary, Sed & 1 day exercise, 1d Ex). Exercise groups were trained by using a 6h/day low intensity swimming program. The expressions of GLUT4, PGC-1α and PPARδ were increased in the 1d Ex group from the Sed group. PPARδ was overexpressed using electric pulse-mediated gene transfer technique in PGC-1α KO mice to confirm effects of PPARδ for GLUT4 biogenesis without PGC-1α. The expressions of GLUT4 and MEF2 were increased in PPARδ overexpressed muscles. These findings suggest that PPARδ-driven GLUT4 biogenesis through MEF2 by acute endurance exercise was independent of PGC-1α. |
| Key words:
Acute Endurance Exercise, GLUT4 biogenesis, PGC-1α, PPARδ, MEF2 |
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