The effect of two-week resistance and endurance training on skeletal muscle repair and regeneration after ischemia-reperfusion in rat

Lim, Chang Hyun; Kim, Chang Keun; Lim, Chang Hyun; Kim, Chang Keun

Chang Hyun Lim, Chang Keun Kim

Department of exercise physiology, Korea National Sport University, Seoul, Korea

Correspondence

Chang Keun Kim , Tel: 02-410-6815, Fax: 02-410-6945, Email: ckkim@knsu.ac.kr

Received: October 23, 2016; Accepted: December 6, 2016. Published online: December 6, 2016.

ABSTRACT

PURPOSE:
The present study examined the effect of two-week resistance and endurance training on skeletal muscle repair and regeneration after ischemia-reperfusion (IR) injury in rat.
METHODS:
Ten week-old male Sprague-Dawley (SD) rats were divided into three groups (IR-CON, IR-RE, IR-EN). To evaluate different property of muscle fiber types, soleus (SOL) and extensor digitorum longus (EDL) muscle were dissected. We conducted confocal microscope and western blot analysis for evaluating cell cycle of skeletal muscle reapir and regeneration.
RESULTS:
Satellite cells (SC) of SOL and EDL muscle were markedly increased after both exercise training. However myonuclear was only increased on SOL muscle. MyoD protein expression in SOL and EDL muscle was increased after endurance exercise training.
CONCLUSIONS:
It is consider that exercise training after IR injury may affect to repair and regeneration of damaged skeletal muscle positively by increasing SC and myonuclear in all exercise training, despite a little different response follow muscle types.

Key words: Ischemia-reperfusion; satellite cell; myonuclear; exercise training; skeletal muscle; 혈류차단-재 관류