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Exerc Sci > Volume 21(4); 2012 > Article
Exercise Science 2012;21(4): 435-443. doi: https://doi.org/10.15857/ksep.2012.21.4.435
지구성 운동 시 NT-PGC-1α가 흰쥐 골격근 내 미토콘드리아 생합성에 미치는 영향
김상현1, 정수련2, 김기진1
Role of NT-PGC-1α on endurance exercise induced mitochondiral biogenesis in rat skeletal muscle.
N-truncated peroxisome proliferator-activated receptor γ coactivator-1α splice variant (NT-PGC-1α) has similar biological effects to peroxisome proliferator-activated receptor γ coactivator-1α(PGC-1α) in brown adipose tissue. But, the effect of NT-PGC-1α on mitochondrial biogenesis in skeletal muscles has not been clearly identified. The purpose of this study is to verify that NT-PGC1 induce the mitocondrial biogenesis in skeletal muscle. Eighteen rats were randomly assigned into 3 groups (Sedentary, Sed; 1 day exercise, 1d Ex; 3 day exercise, 3d Ex). Exercise groups were trained by using a 6 h/day swimming program. Muscle samples for PGC-1α, NT-PGC-1α and mitochondrial enzymes were harvested 18 hours after the last bout of exercise. The expression of PGC-1α was increased in the 1d Ex compared with Sed group, but 3d Ex group was not changed compared with Sed group. NT-PGC-1α was increased in the 3d Ex group from the Sed group. The expressions of cytochrome oxidase subunit I (COXⅠ)and NADH-ubiquinone oxidoreductase (NADH-UO) were increased in the 1d Ex and 3d Ex group from the Sed group. Three day overexpressed NT-PGC-1α C2C12 myotubes were harvested for mitochondrial enzymes. The expressions of mitochondrial enzymes, cytochrome c, COXⅠ, ATP synthase and citrate synthase were increased in the NT-PGC-1α overexpressed C2C12 from the Sed group. These findings suggest that PGC-1α protein is responsible for initial increase in mitochondrial biogenesis. While NT-PGC-1α is important for chronic exercise induced increase in mitochondrial proteins.
Key words: PGC-1α, NT-PGC-1α, skeletal muscle, C2C12, endurance exercise, mitochondrial biogenesis
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