| PPARβ/δ 과발현은 PGC-1α ubiquitination의 감소를 통해 PGC-1α
단백질을 증가시킨다 |
| 고진호1, 김홍수2, 김기진3 |
1Washington University School of Medicine
2계명문화대학교
3계명대학교 |
| PPARβ/δOverexpression Increase PGC-1αProtein through the Decreased PGC-1α Ubiquitination
in Mouse Skeletal Muscle. |
| Jin-Ho Koh1, Hong-Soo Kim2, Ki-Jin Kim3 |
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| Correspondence |
Ki-Jin Kim , Tel: +82-53-580-5256, Fax: +82-54-580-5314, Email: kjk744@kmu.ac.kr
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Received: September 30, 2015; Accepted: November 20, 2015. Published online: November 30, 2015. |
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| ABSTRACT |
PURPOSE:
The aim of this study was to find mechanism with which PPARβ/δ control PGC-1αprotein stability in mouse skeletal muscle.
METHODS:
Tibialis anterior (TA) muscles of rat were dissected at 18h after 2 weeks swimming exercise. PPARβ/δ was over-expressed in
mouse tibialis anterior (TA) muscle using electrical pulse-mediated gen transfer (electroporation; EPO) method. To evaluate, PGC-1α mRNA
and protein, PPARβ/δ, ubiquitin and mitochondrial enzyme protein expression in mouse skeletal muscle, TA muscle were dissected at 2wk
and 4wk after EPO. To evaluate binding between PGC-1α and ubiquitin, we conducted V5-PGC-1α Immunoprecipitation.
RESULTS:
PPARβ/δ and PGC-1α in swimming skeletal muscle for 2 weeks were increased 2.1 and 2.5 fold respectively than sedentary
muscle. PGC-1α mRNA in PPARβ/δ over-expression skeletal muscle were not different at 2 wk and 4wk when compared to an empty vector
(EV) treated group. Endogenous and exogenous PPARβ/δ in PPARβ/δ over-expression skeletal muscle were increased 2.5 and 3.8 fold
respectively when compared to an EV treated group. We identified binding between PGC-1α and ubiquitin, and ubiquitin were decreased
60% in PPARβ/δ transfected C2C12 when compared to an EV treated group. PGC-1α were increased 3 fold in muscle that ubiquitin were
decreased 60% when compared to an EV treated muscle and COXI and CYTO C were increased 2.27 and 1.75 fold respectively in muscle
when compared to an EV treated muscle.
CONCLUSIONS:
PPARβ/δ overexpression increases PGC-1α protein through the decreased PGC-1α ubiquitination without the increased
PGC-1α mRNA in mouse skeletal muscle. |
| Key words:
endurance exercise, PPARβ/δ, PGC-1α, ubiquitin, mitochondrial enzymes |
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