The Effect of Endurance Exercise Training on Inflammatory Cytokines, Apoptosis, and FOXO Expression in Adipose Tissue of Hypertensive Rats

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doi:2014.23.2.89

Exerc Sci > Volume 23(2); 2014 > Article

Exercise Science 2014;23(2): 89-98. doi: https://doi.org/10.15857/ksep.2014.23.2.89

지구성운동이 고혈압 동물의 지방조직 내 염증, 세포사멸 및 FOXO 발현에 미치는 효과

이영란¹, 박희근¹, 장학영¹, 김명화², 이상기¹

¹충남대학교
²우송대학교

The Effect of Endurance Exercise Training on Inflammatory Cytokines, Apoptosis, and FOXO Expression in Adipose Tissue of Hypertensive Rats

ABSTRACT

The purpose of this study was to investigate the expression of p53, FOXO1, FOXO3a, inflammatory cytokines and apoptosis in adipose tissue of endurance exercise trained rats. The Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) were divided into 3 groups: WKY control (WKY, n=9), SHR control (SHR-C, n=9), SHR exercise (SHR-E, n=9), and submitted to treadmill exercise for 16 weeks (16 m/min, 5 days/week, 30-60 min/day). Every other weeks, systolic blood pressure were measured. After the last bout of exercise, all rats were sacrificed, then, p53, FOXO1, and FOXO3a were analyzed by western blotting. Also, inflammatory cytokines (IL-6, TNF-α, MCP-1) and apoptotic proteins(Bax, Caspase-9, and Caspase-3) were analyzed by RT-PCR. The expression of inflammatory cytokines (TNF-α, MCP-1) and apoptosis markers (Bax, Caspase-9, and Caspase-3) were significantly decreased in SHR-E compared to the SHR-C. Additionally, p53 was significantly decreased and FOXO1 was significantly increased by exercise in SHR rats. These findings suggest that endurance exercise may decreases systolic blood pressure via enhancement of FOXO1 and suppression of inflammatory cytokines (TNF-α, MCP-1) and apoptosis factors (p53, Bax, Caspase-9, and Caspase-3) in adipose tissue of SHRs.

Key words: hypertension, aerobic exercise, p53, FOXO, inflammatory cytokine, apoptosis