The effects of glycogen supercompensation by single bout of glycogen depletion exercise with normal diet in untrained rat skeletal muscle

,; ,; ,; ,; ,; ,; ,; ,

doi:2013.22.2.143

Exerc Sci > Volume 22(2); 2013 > Article

Exercise Science 2013;22(2): 143-150. doi: https://doi.org/10.15857/ksep.2013.22.2.143

트레이닝 경험이 없는 흰쥐의 1회 글리코겐 고갈 운동에 따른 탄수화물 초과 보상 효과

고진호¹, 김기진¹, 정수련², 김상현¹

¹계명대학교
²경주대학교

The effects of glycogen supercompensation by single bout of glycogen depletion exercise with normal diet in untrained rat skeletal muscle

ABSTRACT

The purpose of this study was to examine if excessive glycogen accumulation can be achieved with single bout of submaximal exercise in untrained rat skeletal muscle without diet manipulation. Sixteen rats were randomly divided into 2 group(control & single bout endurance exercise group). Animals were performed 6 hour long swimming with normal chow diet. Glycogen concentrations were increased 2.3 fold(p<.001) in exercised muscles. The expression of GLUT-4 protein expressions were 2.5 fold(p<.001) higher than control group, but hexokinase II protein expressions were not changed by single bout of endurance exercise. The expression and activation of AMPK in swim exercised muscles were 2.9 fold(p<.001) and 3.1 fold(p<.05) higher in trained muscles. These observation leads us to conclude that single bout of glycogen depleting submaximal exercise with normal diet is more than enough to induce glycogen supercompensation. The glycogen supercompensation may be influenced by increased induction of GLUT-4 protein and activation of AMPK.

Key words: Glycogen supercompensation, Endurance exercise, Skeletal muscle, Glycogen, AMPK, GLUT-4, HK II