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Exerc Sci > Volume 21(2); 2012 > Article
Exercise Science 2012;21(2): 223-230. doi: https://doi.org/10.15857/ksep.2012.21.2.223
유산소 운동수행이 흰쥐 골격근의 면역관련 TLR4 단백질의 신호전달 활성에 미치는 영향
여효성, 김효정, 김창근
Effect of endurance exercise on TLR4 signaling-mediated protein expression in rats
The innate immune response is the first line of defence against infectious disease. A group of proteins that comprise Toll or Toll-like family of receptors (TLRs) has been suggested as one of the markers associated with immune and inflammatory responses. TLR4 among the TLRs produces a response by lipopolysaccharide (LPS) and controls inflammatory responses. This study was performed to demonstrate the change of TLR4 signaling-mediated protein expressions in skeletal muscle during the recovery of an intensive endurance exercise. Sprague-Dawley rats (12 week, n=35) were randomly divided into five groups(n=7 each) to time period of the exercise (pre, post 0 h, 30min, 1 hr and 6 hrs). The rats ran on the rodent treadmill for 1 hour at the level of 75~80%V̇O2max (28 m/min). The change of TLR4, MyD88 protein expression and NFκBp65 DNA binding activity were analyzed by western blotting in Extensor Digitorum Longus (EDL) muscle. TLR4 protein expression was significantly decreased by 41%(p=.036) at 6hrs of recovery with the intensive endurance exercise whereas MyD88 was not changed. NFκB was significantly increased by 51%(p=.014) at 1h of recovery but returned to the resting level at 6 hrs of recovery. These results suggest a possibility of an interaction between TLR4and NFκB expression and the exercise-induced changes in these molecules during recovery period may a key role to defence inflammatory response in rat skeletal muscle.
Key words: TLR4, NFκB, Endurance exercise, Inflammatory response
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