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Exerc Sci > Volume 12(4); 2003 > Article

Exercise Science 2003;12(4): 641-652.

장기간의 유산소 훈련과 Rotenone 투여가 Glycogen 및 효소 활성 변화에 미치는 영향

진화은, 백일영

연세대학교

ABSTRACT

Jin, H.E., Paik, I.Y. The Effect of Aerobic Training and Rotenone Supplementation on Glycogen and Enzyme activity. Exercise Science, 12(4): 641-652, 2003. The purpose of this study was to investigate the effects of a swimming training and rotenone supplement on level of glycogen and enzyme activity in SD(Sprague-Dawley). Sixty male SD were divided into three groups consisting of control group(C), rotenone supplement group(R), rotenone supplement and training group(RT). Training groups participated in a regular swimming training, and supplement groups were fed rotenone. At the end of the trial, the animal was killed and muscle and liver were taken and analyzed as glycogen, citrate synthase(CS) and β-hydroxyacyl CoA dehydrogenase(β-HAD). The obtained results were analyzed via three-way ANOVA using SPSS program. A value of p<.05 was considered statistically significant. The level of glycogen concentration in gastrocnemius, soleus and liver was decreased with exercise(p<.05), and in resting, RT was showed to be the highest level among groups. The level of CS activity in gastrocnemius, soleus was increased with exercise and the order of resting CS activity was RT, C, R(p<.05). The level of β-HAD activity in gastrocnemius, soleus and liver was elevated with exercise except group C in liver(p<.05).In conclusion rotenone supplementation inhibited complex Ⅰ of electron transport chain(ETC) decreased level of glycogen and enzyme activity. Also, during aerobic swim training, energy substrate transport to skeletal muscle and oxidative capacity were increased, so glycogen stores in tissues and enzyme activity increased. And, despite rotenone supplementation, the result of this study showed energy substrate and enzyme activity increased.

Key words: Citrate Synthase(CS), electron trnasport chain(ETC) complex Ⅰ, glycogen, β-Hydroxyacyl CoA Dehydrog -enase(β-HAD), rotenone