| 운동 중 골격근 내 PGC-1α 농도는 PPARβ/δ를 통한 후전사 기전에 의하여 조절된다: PPARβ/δ silence가 ubiquitin을 통한 PGC-1α 안정성에 미치는 영향 |
| 정수련1), 김기진1), 고진호2)* |
1계명대학교
2Washington University School of Medicine |
| PGC-1α concentration in skeletal muscle by exercise is controlled by PPARβ/δ through post-transcriptional mechanism: the effects of PPARβ/δ silence on PGC-1α ubiquitination and stability |
| Su-Ryun Jung1), Ki-Jin Kim1), Jin-Ho Koh2)* |
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| Correspondence |
Jin-Ho Koh , Tel: +82-2-970-9539, Fax: +82-2-970-9593, Email: spogion@gmail.com
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Received: June 29, 2015; Accepted: August 21, 2015. Published online: August 30, 2015. |
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| ABSTRACT |
PURPOSE:
The aim of this study was to identified which PPARβ/δ control PGC-1α protein stability in rat skeletal muscle by long-term endurance exercise.
METHODS:
shPPARβ/δ was over-expressed in rat epitrochlearis (Epi) muscle using electrical pulse-mediated gen transfer (electroporation;EPO) method. Then animal underwent 2 wk long swimming exercise. To evaluate PPARβ/δ, PGC-1α ubiquitination, PGC-1α and mitochondrial enzyme expression in rat skeletal muscle, Epi muscles were dissected 18 h post final bout of swimming exercise.
RESULTS:
PPARβ/δ, PGC-1α, COX I and NADH protein expression in shPPARβ/δ over-expression sedentary (Sed) skeletal muscle were significantly decreased over 55%, but PGC-1α ubiquitination was significantly increased 2.6 fold when compared to scramble (Scr) treated Sed muscle. PPARβ/δ, PGC-1α, COX I and NADH protein expression in 2 wks swimming exercise with Scr gene treated muscle were significantly increased over 2 fold, but PGC-1α ubiquitination was significantly decreased 66% when compared to Scr gene treated Sed muscle. PPARβ/δ, PGC-1α, COX I and NADH protein expression in 2 wks swimming exercise with shPPARβ/δ gene treated muscle were not increased, but PGC-1α ubiquitination was significantly increased 1.96 fold when compared to Scr gene treated Sed muscle.
CONCLUSIONS:
Our results indicate that PPARβ/δ controls PGC-1α protein stability in rat skeletal muscle following long-term endurance exercise. |
| Key words:
long-term endurance exercise, PARβ/δ, PGC-1α, ubiquitin, mitochondrial enzymes |
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