Effect of Acute Exercise on Phosphorylation of ERK and injury in Murine Lung

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doi:2007.16.1.23

Exerc Sci > Volume 16(1); 2007 > Article

Exercise Science 2007;16(1): 23-30. doi: https://doi.org/10.15857/ksep.2007.16.1.23

일회성 트레드밀 운동이 쥐의 폐조직 ERK 인산화와 손상에 미치는 영향

이광희¹, 박성태², 서상훈³, 전태원¹, 전병환⁴

¹서울대학교
²경주대학교
³연세대학교
⁴아산생명과학연구소

Effect of Acute Exercise on Phosphorylation of ERK and injury in Murine Lung

ABSTRACT

Lee, K.H., Park, S.T., Suh, S.H., Jun, T.W., Jeon, B.H. Effect of Acute Exercise on Phosphorylation of ERK and injury in Murine Lung. Exercise Science, 16(1): 23-30, 2007. The purpose of this study was to investigate the effects of acute exercise on phosphorylation of ERK (extracellular signal-regulated kinase) and injury in murine lung. For this study, 7 week-old C57BL/6 mice were used, and they(n=8 per group) were randomly divided into five different experimental groups[1) control 2) low intensity exercise, 3) high intensity exercise, 4) normal pressure mechanical ventilation, and 5) high pressure mechanical ventilation]. Lung ERK1/2 phosphorylation was examined at 0, 15, 30, and 60 minutes, and compared with those of both skeletal and cardiac muscles after C57BL/6 mice ran on the treadmill for 60 minutes. In addition, lung injury after an acute exercise bout was studied, and compared with that by mechanical ventilation to see whether an acute exercise causes lung injury in mice. As a result, acute treadmill exercise significantly increased ERK1/2 phosphorylation in both skeletal muscle and lung tissuues during exercise at 30 minutes(P<0.05). However, there were no significant differences in lung ERK1/2 phosphoyaltion between low- and high-intensity exercise(P>0.05). Mechanical ventilation significantly increased lung ERK1/2 phosphoyaltion(P<0.05). However, there were no significant differences in lung ERK1/2 phosphoyaltion between normal and high pressure mechanical ventilation(P>0.05). Furthermore, high pressure mechanical ventilation induced lung injury, but lung injury was not observed by high-intensity treadmill exercise. These results suggest that acute treadmill running increases ERK 1/2 phosphorylation without the risk of lung tissue being damaged in murine.

Key words: acute exercise, signaling pathway, ERK, mechanical ventilation, exertion