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Exerc Sci > Volume 20(4); 2011 > Article
Exercise Science 2011;20(4): 359-366. doi: https://doi.org/10.15857/ksep.2011.20.4.359
단기간과 장기간 지구성운동 시 쥐의 골격근 내 항산화 효소와 미토콘드리아 효소의 발현양상
김상현, 안나영, 홍창배, 김기진
The effect of acute and prolonged endurance swim exercise on antioxidant and mitochondrial enzymes in rat skeletal muscle.
The majority of the oxidants produced in muscles are due to an elevated rate of mitochondrial respiration. In general, expression of antioxidant enzymes for protection of oxidative stress were increased in both high intensity and long duration exercise. However, there are no studies reported regarding the effect of acute and prolonged endurance exercise on antioxidant enzymes expression. This study measured antioxidant enzymes to evaluate the total antioxidant defense capacity in rat skeletal muscles subjected to acute and prolonged endurance exercise. Twenty-four rats were randomly assigned into 4 groups (sedentary, 1 day exercise, 3 day exercise, 3 weeks exercise). Exercise groups were trained by using a 6 h/day swimming program. Blood samples for TBARS were drawn immediately after the exercise session. Muscles samples for antioxidant and mitochondrial enzymes were harvested 18 hours after the last bout of exercise. Endurance exercise training increased serum TBARS levels in 1 day exercise group, but that of 3 day and 3 weeks exercise groups were not changed compare to sedentary group. The expression of antioxidant enzymes were not changed (catalase, SOD2) or decreased (SOD1, GPx-1) in the muscles' of 1 day exercise group, but SOD1, catalase and SOD2 expression were increased in the 3 day exercise group. Cytoplasmic antioxidant enzymes (SOD1, catalase, GPx-1) were not changed from the sedentary group, but the mitochondrial antioxidant enzyme SOD2 was increased in the muscles' of 3 week exercise group. NADH-UO and COX1 expression were increased in all exercised groups (1 day, 3 day, 3 weeks). Mitochondrial electron transport chain enzymes expression were higher in 3 week group compare to 1 day exercise group. Krebs cycle enzyme citrate synthase only increased after 3 weeks exercise. These results indicate that antioxidant defense capacity by cytoplasmic antioxidant enzymes in muscles' of endurance trained rat were only increased in short term exercise. However, an increase in antioxidant defense capacity by mitochondrial antioxidant enzyme, SOD 2, was increased after prolonged endurance exercise training.
Key words: Acute endurance exercise, Prolonged endurance exercise, Skeletal muscle, Mitochondrial biogenesis, Antioxidant enzyme, TBARS, Oxidative stress
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